論文 / Publications

• Terai Y, Yada E, Nakanishi H, Itaka K. mRNA-Based Combination Therapy for Inflammation-Driven Osteoarthritis Induced by Monosodium Iodoacetate. Pharmaceutics 17(10):1254, 2025

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• Nagaoka K, Nakanishi H, Tanaka H, Anindita J, Kawamura T, Tanaka T, Yamashita T, Kuroda A, Nomura S, Akita H, Itaka K, Kodama T, Kakimi K. Neoantigen mRNA vaccines induce progenitor-exhausted T cells that support anti-PD-1 therapy in gastric cancer with peritoneal metastasis. Gastric Cancer 28(5):825-836, 2025

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• Nakanishi H, Itaka K. Extracellular ligand-responsive translational regulation of synthetic mRNAs using engineered receptors. NPG Asia Materials 17:25, 2025

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• Du X, Nakanishi H, Yamada T, Sin Y, Minegishi K, Motohashi N, Aoki Y, Itaka K. Polyplex Nanomicelle-Mediated Pgc-1α4 mRNA Delivery Via Hydrodynamic Limb Vein Injection Enhances Damage Resistance in Duchenne Muscular Dystrophy Mice. Adv Sci. 2025

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• Yang T, Nakanishi H, Itaka K. Development of a new caged intein for multi-input conditional translation of synthetic mRNA. Sci Rep. 14(1):9988, 2024

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• Du X, Yada E, Terai Y, Takahashi T, Nakanishi H, Tanaka H, Akita H, Itaka K. Comprehensive Evaluation of Lipid Nanoparticles and Polyplex Nanomicelles for Muscle-Targeted mRNA Delivery. Pharmaceutics 15(9): 2291, 2023

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• Zhang M, Fukushima Y, Nozaki K, Nakanishi H, Deng J, Wakabayashi N, Itaka K. Enhancement of bone regeneration by coadministration of angiogenic and osteogenic factors using messenger RNA. Inflammation and Regeneration 43:32, 2023

 

• Free K, Nakanishi H, Itaka K. Development of Synthetic mRNAs Encoding Split Cytotoxic Proteins for Selective Cell Elimination Based on Specific Protein Detection. Pharmaceutics 15(1): 213, 2023
   

• Cui J, Shibata Y, Itaka K, Zhou J, Zhang J. Unbiased comparison and modularization identify time-related transcriptomic reprogramming in exercised rat cartilage: Integrated data mining and experimental validation. Front Physiol. 13: 974266, 2022
   

• Deng J, Fukushima Y, Nozaki K, Nakanishi H, Yada E, Terai Y, Fueki K, Itaka K. Anti-Inflammatory Therapy for Temporomandibular Joint Osteoarthritis Using mRNA Medicine Encoding Interleukin-1 Receptor Antagonist. Pharmaceutics 14(9): 1785, 2022
   

• Kida H, Feril LB, Irie Y, Endo H, Itaka K, Tachibana K. Influence of Nanobubble Size Distribution on Ultrasound-Mediated Plasmid DNA and Messenger RNA Gene Delivery. Front Pharmacol. 13: 855495, 2022
   

• Hashimoto Y, Kuniishi H, Sakai K, Fukushima Y, Du X, Yamashiro K, Hori K, Imamura M, Hoshino M, Yamada M, Araki T, Sakagami H, Takeda S, Itaka K, Ichinohe N, Muntoni F, Sekiguchi M, Aoki Y. Brain Dp140 alters glutamatergic transmission and social behaviour in the mdx52 mouse model of Duchenne muscular dystrophy. Prog Neurobiol. 216: 102288, 2022
   

• Pezzotti G, Zhu W, Terai Y, Marin E, Boschetto F, Kawamoto K, Itaka K. Raman spectroscopic insight into osteoarthritic cartilage regeneration by mRNA therapeutics encoding cartilage-anabolic transcription factor Runx1. Mater Today Bio 13: 100210, 2022
   

• Nakanishi H, Yoshii T, Tsukiji S, Saito H. A protocol to construct RNA-protein devices for photochemical translational regulation of synthetic mRNAs in mammalian cells. STAR Protoc. 3(2): 101451, 2022
   

• Nakanishi H, Saito H, Itaka K. Versatile Design of Intracellular Protein-Responsive Translational Regulation System for Synthetic mRNA. ACS Synth Biol. 11(3): 1077-1085, 2022
   

• Oyama N, Kawaguchi M, Itaka K, Kawakami S. Efficient Messenger RNA Delivery to the Kidney Using Renal Pelvis Injection in Mice. Pharmaceutics 13(11): 1810, 2021
   

• Nakanishi H, Yoshii T, Kawasaki S, Hayashi K, Tsutsui K, Oki C, Tsukiji S, Saito H. Light-controllable RNA-protein devices for translational regulation of synthetic mRNAs in mammalian cells. Cell Chemical Biology 28(5): pp662-674.e5, 2021
   

• Fukushima Y, Uchida S, Imai H, Nakatomi H, Kataoka K, Saito N, Itaka K. Treatment of ischemic neuronal death by introducing brain-derived neurotrophic factor mRNA using polyplex nanomicelle. Biomaterials 270: 120681, 2021
   

• Li J, Sasaki N, Itaka K, Terpstra M, Levato R, Matsusaki M. Regulation of Chondrocyte Differentiation by Changing Intercellular Distances Using Type II Collagen Microfibers. ACS Biomaterials Science & Engineering. 6 (10), 5711-5719, 2020
   

• Uchida S, Yanagihara K, Matsui A, Kataoka K, Itaka K. mRNA as a Tool for Gene Transfection in 3D Cell Culture for Future Regenerative Therapy. Micromachines 11(4): 426, 2020
   

• Crowley ST, Fukushima Y, Uchida S, Kataoka K, Itaka K. Enhancement of Motor Function Recovery after Spinal Cord Injury in Mice by Delivery of Brain-Derived Neurotrophic Factor mRNA. Mol Ther Nucleic Acids 17: 465-476, 2019

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• Lin CY, Crowley S, Uchida S, Komaki Y, Kataoka K, Itaka K. Treatment of intervertebral disk disease by administration of messenger RNA encoding a cartilage-anabolic transcription factor. Mol Ther Nucleic Acids 16: 162-171, 2019
   

• Crowley S, Kataoka K, Itaka K. Combined CatWalk Index: an improved method to measure mouse motor function using the automated gait analysis system. BMC Research Notes 11:263, 2018
   

• Yanagihara K, Uchida S, Ohba S, Kataoka K, Itaka K. Treatment of Bone Defects by Transplantation of Genetically Modified Mesenchymal Stem Cell Spheroids. Molecular Therapy - Methods & Clinical Development. 9: 358-366, 2018

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• Matsui A, Uchida S, Hayashi A, Kataoka K, Itaka K. Prolonged engraftment of transplanted hepatocytes in the liver by transient pro-survival factor supplementation using ex vivo mRNA transfection. J Control Release. 285; 1-11, 2018
   

• Uchida S, Yoshinaga N, Yanagihara K, Yuba E, Kataoka K, Itaka K. Designing immunostimulatory double stranded messenger RNA with maintained translational activity through hybridization with poly A sequences for effective vaccination. Biomaterials. 150:162-170, 2018​​

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• Anraku Y, Kuwahara H, Fukusato Y, Mizoguchi A, Ishii T, Nitta K, Matsumoto Y, Toh K, Miyata K, Uchida S, Nishina K, Osada K, Itaka K, Nishiyama N, Mizusawa H, Yamasoba T, Yokota T, Kataoka K. Glycaemic control boosts glucosylated nanocarrier crossing the BBB into the brain. Nature Communications 8: 1001, 2017​​

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• Perche F, Uchida S, Akiba H, Lin CY, Ikegami M, Dirisala A, Nakashima T, Itaka K, Tsumoto K, Kataoka K. Improved Brain Expression of Anti-Amyloid β scFv by Complexation of mRNA Including a Secretion Sequence with PEG-based Block Catiomer. Curr Alzheimer Res. 14(3):295-302. 2017
   

• Lin CY, Perche F, Ikegami M, Uchida S, Kataoka K, Itaka K. Messenger RNA-based therapeutics for brain diseases: An animal study for augmenting clearance of beta-amyloid by intracerebral administration of neprilysin mRNA loaded in polyplex nanomicelles. J Control Release. 235:268-75, 2016
   

• Uchida S, Hayakawa K, Ogata T, Tanaka S, Kataoka K, Itaka K. Treatment of spinal cord injury by an advanced cell transplantation technology using brain-derived neurotrophic factor-transfected mesenchymal stem cell spheroids. Biomaterials. 109:1-11, 2016
   

• Uchida H, Itaka K, Uchida S, Ishii T, Suma T, Miyata K, Oba M, Nishiyama N, Kataoka K. Synthetic Polyamines to Regulate mRNA Translation through the Preservative Binding of Eukaryotic Initiation Factor 4E to the Cap Structure. J Am Chem Soc. 138(5):1478-81, 2016
   

• Uchida S, Kinoh H, Ishii T, Matsui A, Tockary TA, Takeda KM, Uchida H, Osada K, Itaka K, Kataoka K. Systemic delivery of messenger RNA for the treatment of pancreatic cancer using polyplex nanomicelles with a cholesterol moiety. Biomaterials. 82:221-8, 2016
   

• Aini H, Itaka K, Fujisawa A, Uchida H, Uchida S, Fukushima S, Kataoka K, Saito T, Chung U, Ohba S. Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment. Sci Rep. 6: 18743, 2016
   

• Okuda T, Suzuki Y, Kobayashi Y, Ishii T, Uchida S, Itaka K, Kataoka K, Okamoto H. Development of Biodegradable Polycation-Based Inhalable Dry Gene Powders by Spray Freeze Drying. Pharmaceutics. 7(3): 233-54, 2015
   

• Matsui A, Uchida S, Ishii T, Itaka K, Kataoka K. Messenger RNA-based therapeutics for the treatment of apoptosis-associated diseases. Sci Rep. 5:15810, 2015
   

• Li Y, Osada K, Chen Q, Tockary TA, Dirisala A, Takeda KM, Uchida S, Nagata K, Itaka K, Kataoka K. Toroidal Packaging of pDNA into Block Ionomer Micelles Exerting Promoted in Vivo Gene Expression. Biomacromolecules. 16(9): 2664-71, 2015
   

• Itaka K, Uchida S, Matsui A, Yanagihara K, Ikegami M, Endo T, Ishii T, Kataoka K. Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation. J Vis Exp. 101: e52384, 2015
   

• Uchida S, Kataoka K, Itaka K. Screening of mRNA Chemical Modification to Maximize Protein Expression with Reduced Immunogenicity. Pharmaceutics. 7(3):137-51, 2015
   

• Chen Q, Osada K, Pennisi M, Uchida S, Tockary TA, Dirisala A, Li Y, Takeda KM, Oniyanagi S, Itaka K, Kataoka K. A tadpole-shaped gene carrier with distinct phase segregation in a ternary polymeric micelle. Soft Matter. 11(14):2718-22, 2015
   

• Baba M, Itaka K, Kondo K, Yamasoba T, Kataoka K. Treatment of neurological disorders by introducing mRNA in vivo using polyplex nanomicelles. J Control Release. 201:41-8，2015
   

• Hayakawa K, Uchida S, Ogata T, Tanaka S, Kataoka K, Itaka K. Intrathecal injection of a therapeutic gene-containing polyplex to treat spinal cord injury. J Control Release. 197:1-9，2015
   

• Uchida H, Itaka K, Nomoto T, Ishii T, Suma T, Ikegami M, Miyata K, Oba M, Nishiyama N, Kataoka K.　Modulated protonation of side chain aminoethylene repeats in N-substituted polyaspartamides promotes mRNA transfection. J Am Chem Soc. 136(35):12396-405, 2014
   

• Dirisala A, Osada K, Chen Q, Tockary TA, Machitani K, Osawa S, Liu X, Ishii T, Miyata K, Oba M, Uchida S, Itaka K, Kataoka K. Optimized rod length of polyplex micelles for maximizing transfection efficiency and their performance in systemic gene therapy against stroma-rich pancreatic tumors. Biomaterials. 35(20):5359-68, 2014
   

• Nagata K, Itaka K, Baba M, Uchida S, Ishii T, Kataoka K. Muscle-targeted hydrodynamic gene introduction of insulin-like growth factor-1 using polyplex nanomicelle to treat peripheral nerve injury. J Control Release. 20;183C:27-34, 2014
   

• Ge Z, Chen Q, Osada K, Liu X, Tockary TA, Uchida S, Dirisala A, Ishii T, Nomoto T, Toh K, Matsumoto Y, Oba M, Kano MR, Itaka K, Kataoka K. Targeted gene delivery by polyplex micelles with crowded PEG palisade and cRGD moiety for systemic treatment of pancreatic tumors. Biomaterials. 35(10):3416-26, 2014
   

• Uchida S, Itaka K, Nomoto T, Endo T, Matsumoto Y, Ishii T, Kataoka K. An injectable spheroid system with genetic modification for cell transplantation therapy. Biomaterials. 35(8):2499-506, 2014
   

• Izumida Y, Yahagi N, Takeuchi Y, Nishi M, Shikama A, Takarada A, Masuda Y, Kubota M, Matsuzaka T, Nakagawa Y, Iizuka Y, Itaka K, Kataoka K, Shioda S, Niijima A, Yamada T, Katagiri H, Nagai R, Yamada N, Kadowaki T, Shimano H. Glycogen shortage during fasting triggers liver-brain-adipose neurocircuitry to facilitate fat utilization. Nat Commun. 4:2316, 2013
   

• Theofilus A. Tockary, Kensuke Osada, Qixian Chen, Kaori Machitani, Anjaneyulu Dirisala, Satoshi Uchida, Takahiro Nomoto, Kazuko Toh, Yu Matsumoto, Keiji Itaka, Koji Nitta, Kuniaki Nagayama, and Kazunori Kataoka. Tethered PEG Crowdedness Determining Shape and Blood Circulation Profile of Polyplex Micelle Gene Carriers. Macromolecules 46 (16), 6585?6592, 2013
   

• Ohgidani M, Furugaki K, Shinkai K, Kunisawa Y, Itaka K, Kataoka K, Nakano K. Block/homo polyplex micelle-based GM-CSF gene therapy via intraperitoneal administration elicits antitumor immunity against peritoneal dissemination and exhibits safety potentials in mice and cynomolgus monkeys. J Control Release. 10;167(3):238-47, 2013
   

• Uchida S, Itaka K, Uchida H, Hayakawa K, Ogata T, Ishii T, Fukushima S, Osada K, Kataoka K. In vivo messenger RNA introduction into the central nervous system using polyplex nanomicelle. PLoS One. 8(2):e56220, 2013
   

• Endo T, Itaka K, Shioyama M, Uchida S, Kataoka K. Gene transfection to spheroid culture system on micropatterned culture plate by polyplex nanomicelle: a novel platform of genetically-modified cell transplantation. Drug Deliv. and Transl. Res. 2:398?405, 2012.
   

• Kumagai M, Shimoda S, Wakabayashi R, Kunisawa Y, Ishii T, Osada K, Itaka K, Nishiyama N, Kataoka K, Nakano K. Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination. J Control Release. 160(3):542-51, 2012
   

• Uchida S, Itaka K, Chen Q, Osada K, Ishii T, Shibata MA, Harada-Shiba M, Kataoka K. PEGylated polyplex with optimized PEG shielding enhances gene introduction in lungs by minimizing inflammatory responses. Mol Ther. 20(6):1196-203, 2012.
   

• Chen Q, Osada K, Ishii T, Oba M, Uchida S, Tockary TA, Endo T, Ge Z, Kinoh H, Kano MR, Itaka K, Kataoka K. Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors. Biomaterials. 33:4722-30, 2012
   

• Suma T, Miyata K, Ishii T, Uchida S, Uchida H, Itaka K, Nishiyama N, Kataoka K. Enhanced stability and gene silencing ability of siRNA-loaded polyion complexes formulated from polyaspartamide derivatives with a repetitive array of amino groups in the side chain. Biomaterials. 33: 2770-9, 2012
   

• Osada K, Shiotani T, Tockary TA, Kobayashi D, Oshima H, Ikeda S, Christie RJ, Itaka K, Kataoka K. Enhanced gene expression promoted by the quantized folding of pDNA within polyplex micelles. Biomaterials. 33(1):325-32, 2012
   

• Uchida H, Miyata K, Oba M, Ishii T, Suma T, Itaka K, Nishiyama N, Kataoka K. Odd-Even Effect of Repeating Aminoethylene Units in the Side Chain of N-Substituted Polyaspartamides on Gene Transfection Profiles. J Am Chem Soc. 133(39):15524-32, 2011
   

• Uchida S, Itaka K, Chen Q, Osada K, Miyata K, Ishii T, Harada-Shiba M, Kataoka K. Combination of Chondroitin Sulfate and Polyplex Micelles from Poly(ethylene glycol)- poly{N’-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} Block Copolymer for Prolonged In Vivo Gene Transfection with Reduced Toxicity. J Control Release. 155(2):296-302, 2011
   

• Miyata K, Gouda N, Takemoto H, Oba M, Lee Y, Koyama H, Yamasaki Y, Itaka K, Nishiyama N, Kataoka K. Enhanced transfection with silica-coated polyplexes loading plasmid DNA. Biomaterials. 31(17):4764-70, 2010
   

• Oba M, Vachutinsky Y, Miyata K, Kano MR, Ikeda S, Nishiyama N, Itaka K, Miyazono K, Koyama H, Kataoka K. Antiangiogenic Gene Therapy of Solid Tumor by Systemic Injection of Polyplex Micelles Loading Plasmid DNA Encoding Soluble Flt-1. Mol Pharm. 7(2):501-9, 2010

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• Lee Y, Ishii T, Kim HJ, Nishiyama N, Hayakawa Y, Itaka K, Kataoka K. Efficient Delivery of Bioactive Antibodies into the Cytoplasm of Living Cells by Charge-Conversional Polyion Complex Micelles. Angew Chem Int Ed Engl. 49(14):2552-2555, 2010
   

• Itaka K, Osada K, Morii K, Kim P, Yun SH, Kataoka K. Polyplex nanomicelle promotes hydrodynamic gene introduction to skeletal muscle. J Control Release. 143(1):112-9, 2010
   

• Itaka K, Ishii T, Hasegawa Y, Kataoka K. Biodegradable polyamino acid-based polycations as safe and effective gene carrier minimizing cumulative toxicity. Biomaterials. 31(13):3707-14, 2010
   

• Takeuchi Y, Yahagi N, Izumida Y, Nishi M, Kubota M, Teraoka Y, Yamamoto T, Matsuzaka T, Nakagawa Y, Sekiya M, Iizuka Y, Ohashi K, Osuga JI, Gotoda T, Ishibashi S, Itaka K, Kataoka K, Nagai R, Yamada N, Kadowaki T, Shimano H. Polyunsaturated fatty acids selectively suppress sterol regulatory element-binding protein-1 through proteolytic processing and autoloop regulatory circuit. J Biol Chem. 285(15):11681-91, 2010